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Č. položky:
145283146
Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml
Č. položky:
145283146
€ 57
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*All items will import from USA
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Importované z obchodu USA
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Our biological buffers are essential for protein research, molecular biology, and diagnostics, manufactured in an ISO certified facility for guaranteed quality.
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What Stands Out
Pre-Prepared Solution
This phosphate buffer is ready to use, saving time and effort in preparation, ensuring consistency in experiments and reliability for researchers requiring a stable pH of 9.0.
High Concentration
With a 0.5 M concentration, this buffer provides superior buffering capacity, effectively maintaining pH levels during biochemical reactions, making it ideal for a variety of laboratory applications.
Versatile Applications
Suitable for various biochemical and molecular biology experiments, this buffer facilitates optimal conditions for reactions such as enzyme assays and protein purification, enhancing experimental outcomes.
Detaily produktu
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- Phosphate Buffer, pH 6.5 [0.5 M] Phosphate buffer is used in immunochemical staining procedures and serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4). This buffer is commonly used as a component of irrigation solution during surgeries to preserve the living tissues.
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.78 |
| Included Components | Bottle |
| Item Weight | 8 ounces (226.8 grams) |
| Brand | eCon-Lab-Supply |
What's in the box
Bottle
Who Should Buy?
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Biochemical Research
Ideal for scientists conducting experiments requiring stable pH conditions in biochemical assays and reactions.
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Molecular Biology
Useful for researchers performing DNA/RNA extractions, where maintaining pH is critical to ensure integrity.
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Cell Culture
Beneficial for cell culture applications, particularly those requiring a stable pH for optimal cell growth and function.
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Acidic Environments
Not suitable for applications requiring acidic conditions, as this buffer maintains a pH of 9.0.
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Non-Biological Use
Inappropriate for industrial or non-scientific applications where buffering agents are not required.
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Short Experiments
Not ideal for short-duration experiments where a simple buffer with less complexity is needed.
OPIS PRODUKTU
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Otázky zákazníkov a odpovede na ne
-
otázka:
What is a phosphate buffer and how is it used?
odpoveď: A phosphate buffer is a solution that maintains a stable pH level, specifically at pH 9.0 in this case. It is widely used in biological and biochemical applications, making it essential for experiments requiring a specific pH environment. By resisting changes in acidity or alkalinity, phosphate buffers are ideal for cell culture, enzyme reactions, and various laboratory procedures where pH stability is critical. -
otázka:
Why is a pH of 9.0 significant for phosphate buffers?
odpoveď: A pH of 9.0 is significant because it provides an alkaline environment suitable for various biochemical reactions. Many enzymes and proteins exhibit optimal activity at this pH, making phosphate buffers with this specific pH crucial in research and industry. For example, alkaline phosphatase, an enzyme used in molecular biology, thrives in such conditions, enhancing its functionality in experiments. -
otázka:
How should the phosphate buffer be stored?
odpoveď: Phosphate buffer should be stored in a cool, dry place, away from direct sunlight. Ideally, it should be kept in a tightly sealed, light-resistant container to prevent degradation. Proper storage is essential to maintain its effectiveness and stability, especially if you plan to use it across multiple experimental setups. For extended use, refrigerating can enhance longevity. -
otázka:
Can I use phosphate buffer in my laboratory experiments?
odpoveď: Yes, phosphate buffer is ideal for laboratory experiments, particularly those involving biochemical and biological assays. Its pH stability makes it suitable for cell cultures, DNA manipulation, and enzyme studies. When you're conducting experiments that require consistent environmental conditions, using a phosphate buffer helps ensure the reliability of your results while maximizing enzyme activity and reducing variability. -
otázka:
What are some common applications for phosphate buffers?
odpoveď: Phosphate buffers are commonly used in molecular biology, protein purification, and microbiological culture media. They are essential in applications like western blotting, enzyme assays, and electrophoresis, where maintaining a consistent pH is crucial. By providing a controlled environment, phosphate buffers enhance experimental accuracy and reproducibility, which are paramount in research settings. -
otázka:
Is the phosphate buffer toxic or hazardous?
odpoveď: The phosphate buffer at pH 9.0 is generally considered safe for use in laboratory settings. However, as with any chemical, it should be handled with care. Always utilize personal protective equipment (PPE) such as gloves and safety goggles, and ensure good ventilation. Familiarizing yourself with the material safety data sheet (MSDS) is advisable to understand any specific precautions. -
otázka:
What is the significance of the 0.5 M concentration?
odpoveď: The 0.5 M concentration indicates a moderate buffer capacity, meaning it can effectively resist changes in pH upon the addition of acids or bases. This concentration is particularly useful for experiments requiring reliable pH conditions without overpowering reactions. It allows flexibility in various applications while providing sufficient buffering to maintain a stable environment. -
otázka:
How can I prepare a phosphate buffer at pH 9.0?
odpoveď: To prepare a phosphate buffer at pH 9.0, you'll need to mix disodium hydrogen phosphate (Na2HPO4) and potassium dihydrogen phosphate (KH2PO4) in the correct proportions. By adjusting the amounts and measuring the pH, you can achieve your desired buffer solution. This method allows you to customize the buffer for specific experimental needs while ensuring it meets the pH requirements. -
otázka:
What is the shelf life of this phosphate buffer?
odpoveď: The shelf life of phosphate buffers typically ranges from several months to a year, depending on storage conditions. For optimal preservation, keep the buffer in a cool, dark place and avoid contamination. Always check for any visible signs of degradation before use. Proper storage practices can maintain its effectiveness for a longer duration, ensuring you have a reliable buffer when needed. -
otázka:
Where can I buy Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml?
odpoveď: You can purchase Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml on Ubuy in Slovakia. Ubuy provides a convenient online shopping experience with a wide range of laboratory supplies, making it easy to find the specific buffer requirements for your experiments.
eCon Lab Supply Buffers Editorial Review
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€ 57
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Funkcie a výhody
- Comprehensive range of biological buffers for research and diagnostics.
- Ideal for protein research, molecular biology, and characterization.
- Manufactured in a USA facility certified to ISO 9001 and 13485.
- Produced under stringent Good Manufacturing Practices.
- Each batch undergoes rigorous quality control tests.
- Popular for immunochemical staining and preserving living tissues.